Targeted Mutagenesis of Arabidopsis thaliana using Engineered TAL Effector Nucleases (TALENs)
نویسندگان
چکیده
منابع مشابه
Targeted Mutagenesis of Arabidopsis thaliana Using Engineered TAL Effector Nucleases
Custom TAL effector nucleases (TALENs) are increasingly used as reagents to manipulate genomes in vivo. Here, we used TALENs to modify the genome of the model plant, Arabidopsis thaliana. We engineered seven TALENs targeting five Arabidopsis genes, namely ADH1, TT4, MAPKKK1, DSK2B, and NATA2. In pooled seedlings expressing the TALENs, we observed somatic mutagenesis frequencies ranging from 2-1...
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